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  • br The caspases cascade is groups of proteinases that

    2022-05-22


    The caspases cascade is groups of proteinases that have central roles in the activation of apoptosis. Activation of specific caspase determines the cell death pathways, for instance, activation of caspase-8 indicates the involvement of the extrinsic apoptosis pathway, while activation of caspase-9 suggests the intrinsic apoptosis pathway [35]. Our study however, indicates that 8-PN induces both extrinsic and intrinsic apoptosis pathways in HCT-116 cells through activation of caspase-8 and caspase-9, respectively. Previous study has been reported that 8-PN possess apoptotic properties against MCF-7 cells through activation of caspase-8, whereas intrinsic apoptosis pathway was not investigated [19].
    Although in this study we had proven that 8-PN induces apoptosis in HCT-116 through intrinsic and extrinsic apoptosis pathways, further investigations are required to understand the exact mechanism by which 8-PN acts against HCT-116 at the protein and gene expression levels. Moreover, our data indicate that 8-PN inhibits cell proliferation  Life Sciences 232 (2019) 116633
    in G0/G1 phase, however the signaling pathways of SB 431542 sup-pression is still unclear.
    5. Conclusion
    In conclusion, our results demonstrated that 8-PN induces apoptosis in HCT-116 colon cancer cells. This compound activates both the early and late stages of apoptosis as demonstrated via fluorescence micro-scopy and flow cytometry analysis. Furthermore, 8-PN may trigger both intrinsic and extrinsic apoptosis signaling pathways to cause cell death. In addition, 8-PN also induces cell cycle arrest at G0/G1 phase in HCT-116 cells in a time-dependent manner. Given the potential anticancer effect of 8-PN, further in-depth knowledge about the molecular me-chanisms of cell death induced by 8-PN is urgently required for future research targeting colorectal cancer.
    Funding
    This study was supported by University of Malaya, Malaysia through UMRG grant No. RG336-15AFR.
    Declaration of Competing Interest
    The authors declare that they have no conflict of interest.
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